Cell Culture

BioVyon^TM scaffolds for cell culture
The open pore structure of a BioVyon^TM scaffold offers optimum conditions for encouraging in-vitro cell culture and has been found to offer significant advantages over established Petri dish methods and the ceramic substrates (TCP) traditionally used.

Example 1: Osteoblast culture
Work undertaken by a University has demonstrated that oesteoblast (bone cells) proliferate within a BioVyon^TM scaffold at a comparable rate to standard materials after 4 hours. However, after 72 hours the cells had formed a 3D structure of healthy differentiated cells that exhibited enhanced collagen synthesis and ALP activity. The work concluded that BioVyon^TM scaffolds supported an optimum MG63 human osteoblast adhesion. When confluent, they did not undergo apoptosis but they formed islets of 3D tissue. Initial cell proliferation was more rapid on TCP than BioVyon™ scaffolds but subsequent cell differentiation on BioVyon^TM scaffolds was more rapid and significantly higher than TCP.

Example 2: Stem cell culture
Efficient and accurate differentiation of adult and embryonic stem cells remains an overriding research priority in laboratories around the world. At present, many stem cell differentiation protocols are limited by the non-physiological two-dimensional nature of traditional cell culture methodologies. Extensively optimised differentiation protocols in these traditional culture systems typically generate mixed populations of cells, of which less than five percent may be of the desired adult differentiated phenotype. A collaborative study has demonstrated the use of BioVyon^TM scaffolds as three dimensional biomimetic supports that promote enhanced stem cell growth and differentiation.